I am an employee of the MiraiBio Group of Hitachi Software Engineering.

We developed MasterPlex ReaderFit which is a curve-fitting software specifically designed for ELISA or dose-response analysis. It includes the very popular 4PL nonlinear regression model as well as the 5PL (including weighting options).

I have recently written a blog post on the 4 Parameter Logistic model equation which goes into pretty good details (including lots of images) in the context of ELISA and dose-response analysis.

There is a free fully functional 14-day free trial of MasterPlex ReaderFit. The product page contains a 1:43 minute demo video.

Allen Liu
MiraiBio