To use the "not equal to" or "does not equal" comparison in the IF function, you can use the "<>" operator. Here's an example:

Let's say you have a list of numbers in column A, and you want to check if each number is not equal to 5. You can use the following formula in cell B1:

1. =IF(A1<5, "Not 5", "Is 5")

This formula checks if the value in cell A1 is not equal to 5. If it is not equal to 5, the formula returns "Not 5". If it is equal to 5, the formula returns "Is 5".

You can then drag this formula down to apply it to the rest of the cells in column B, and it will check each corresponding value in column A.