To use the "not equal to" or "does not equal" comparison in an IF function in Excel, you can use the "<" operator. Here's an example:

Let's say you have a list of numbers in column A, and you want to check if each number is not equal to 5. You can use the following formula in cell B1:

1. =IF(A1<5, "Not equal to 5", "Equal to 5")

This formula checks if the value in cell A1 is not equal to 5. If it is not equal to 5, the formula returns "Not equal to 5". If it is equal to 5, the formula returns "Equal to 5".

You can then copy the formula down to the rest of the cells in column B to check the rest of the numbers in column A.