Yes, you can easily insert a vertical line separator in Microsoft Excel. Here's how:

1. First, select the cell or cells where you want to insert the vertical line separator.
2. Next, click on the "Insert" tab in the top menu bar.
3. Look for the "Shapes" option in the "Illustrations" section of the ribbon.
4. Click on the "Shapes" dropdown menu and select the "Line" option.
5. Click and drag your mouse to draw a vertical line where you want it to appear.
6. You can adjust the thickness and style of the line by selecting it and using the formatting options in the "Shape Format" tab that appears in the ribbon.
7. Finally, you can add text to either side of the line by typing it directly into the cells next to the line.

That's it! You should now have a vertical line separator in your Excel worksheet.