If I understand you correctly, then all you need to do is change 3 to 2
in the following row:

Set rng1 = Cells(2, i).Resize(rngA.Rows.Count, 1) ''set range to start
from row 2 instead of 3

But I'm not sure if this really is all that you are after.

Regards,
Steve

PG Oriel wrote:

I have the following code which hides blank columns in my autofilter, but
this was set up to work for rows 3 downawards. I'd like to adapt it to work
in another sheet, starting at row 2. Any ideas? Thanks.

Sub ABC()
Dim lastColumn As Long, rngA As Range, i As Long
Dim rng1 As Range
Application.ScreenUpdating = False
Columns.Hidden = False
lastColumn = Me.Cells(2, "IV").End(xlToLeft).Column
Set rngA = Me.AutoFilter.Range
Set rngA = rngA.Offset(1, 0) _
.Resize(rngA.Rows.Count - 1, 1)
For i = 4 To lastColumn
Set rng1 = Cells(3, i).Resize(rngA.Rows.Count, 1)
If Application.Subtotal(3, rng1) = 0 Then
Columns(i).Hidden = True
End If
Next
Application.ScreenUpdating = True
End Sub