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Thumbs up Answer: Cannot Add Error Bars for individual data points!

It sounds like you are trying to add error bars to individual data points in your Excel column chart, but are having trouble doing so because Excel only allows you to add error bars to a series of data. However, you also need to group your data by subsets (e.g. null vs. dose, 0 hours vs. 24 hours).
  1. Select your chart and click on the "Chart Design" tab in the ribbon.
  2. Click on "Select Data" in the "Data" group.
  3. In the "Select Data Source" dialog box, click on the "Switch Row/Column" button if your data is organized by rows instead of columns.
  4. Click on the "Add" button to add a new series of data.
  5. In the "Edit Series" dialog box, enter a name for your new series (e.g. "Null 0 Hours").
  6. In the "Series X values" field, select the range of cells that contain the X values for this series (e.g. the range of cells that contain the null values for 0 hours).
  7. In the "Series Y values" field, select the range of cells that contain the Y values for this series (e.g. the range of cells that contain the average values for the null values at 0 hours).
  8. Click "OK" to close the dialog box and add the new series to your chart.
  9. Repeat steps 4-8 for each subset of data that you want to group together (e.g. "Null 24 Hours", "Dose 0 Hours", "Dose 24 Hours").
  10. Once you have added all of your series, click on one of the bars in your chart to select the entire series.
  11. Click on the "Chart Design" tab in the ribbon and click on "Add Chart Element" in the "Chart Layouts" group.
  12. Select "Error Bars" and then "More Error Bar Options".
  13. In the "Format Error Bars" pane that appears on the right side of the screen, select "Custom" under "Error Amount".
  14. Click on the "Specify Value" button and then select the range of cells that contain the error values for the selected series (e.g. the range of cells that contain the standard deviation values for the null values at 0 hours).
  15. Click "OK" to close the dialog box and add the error bars to your chart.
  16. Repeat steps 10-15 for each series in your chart.

By adding each subset of data as its own series, you can add error bars to individual data points while still grouping your data by subsets.
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